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KMID : 1094720090140010006
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 1 p.6 ~ p.12
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Effect of Biolinker on the Detection of Prostate Specific Antigen in an Interferometry
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Choi Jung-Min
An Jin-Young
Kim Byung-Woo
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Abstract
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Prostate-specific antigen (PSA) has been identified as a significant biomarker for prostate cancer screening. Heavily-doped porous silicon, etched to form a Fabry-Perot fringe pattern, can be applied to an interferometric sensing for detecting PSA bound with PSA-antibody. In the previous works, a calyx crown derivative (Prolinker-A) was used as an alternative biolinker on the porous silicon surface for interferometric biosensing of DNA-damaging chemical instead of employing the conventional biomolecular affinity method using biotin, which resulted in a denser linker formation. In this study, G5 amine-terminated PAMAM (poly amidoamine) dendrimer as a biolinker, was applied to an interferometric sensing of PSA by using porous silicon, which shows the enhancement of adhesion capability and increase of functional groups more than those with Prolinker-A. Considerably low level down to 1 ng/mL of PSA could be detected by this sensing system.
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KEYWORD
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interferometric biosensor, prostate specific antibody, biolinker, dendrimer, Prolinker-A
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